Vol. 1 No. 3 (2026), Articles
Vol. 1 No. 3 (2026)

EFFECT OF LACTOBACILLUS FERMENTUM ON THE PROTEIN AND AMINO ACID PROFILE OF MILK WHEY AND CURD

Articles
Published 2026-03-11
Xusan Rixsiyev
Kimyo International University in Tashkent
Asilbek Abdullayev
Kimyo International University in Tashkent
Gulmira Mustafayeva
Kimyo International University in Tashkent
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Keywords

curd, whey, total protein, Kjeldahl method, Lowry method, SDS- PAAG, HPLC, amino acid composition.

How to Cite

Rixsiyev , X., Abdullayev , A., & Mustafayeva , G. (2026). EFFECT OF LACTOBACILLUS FERMENTUM ON THE PROTEIN AND AMINO ACID PROFILE OF MILK WHEY AND CURD. WORLD INTERNATIONAL CONFERENCE ON SCIENCE, TECHNOLOGY AND EDUCATION, 1(3), 54-57. https://doi.org/10.5281/zenodo.18948427
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Abstract

This study presents a comprehensive investigation of total protein, soluble protein fractions, and free amino acids in curd and whey obtained by biotechnological and acid methods of milk coagulation. The total protein content was determined using the Kjeldahl method, soluble protein fractions were analyzed by the Lowry method, and the molecular weight distribution of proteins was assessed using SDS- PAAG. The content of free amino acids was determined by high-performance liquid chromatography (HPLC) after derivatization with phenylisothiocyanate. The results demonstrated that fermentation using Lactobacillus fermentum leads to changes in the protein profile and an increase in the concentration of free amino acids in the studied samples.

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References

1. Смирнов, В. В. Молочнокислые бактерии и их использование в пищевой промышленности Москва. 2017.

2. Agilent Technologies. Amino Acid Analysis using Agilent Zorbax Eclipse Plus Columns and the Agilent 1260/1290 Infinity II LC Systems. – Technical Overview, 2022. – Publication No. 5991-8502EN.

3. Bayer, B., et al. SDS-PAGE: Methods and Protocols // Methods in Molecular Biology. – 2024. – Vol. 2735. – P. 3–20.

4. Bidlingmeyer, B. A., et al. (1984). Rapid analysis of amino acids using pre-column derivatization. Journal of Chromatography. (Pico-Tag/PITC метод).